Product Overview

Product description

Highly characterized wild-type mono-nucleosomal (~166 bp) cfDNA for improved QC, preanalytical validation, and analytical validation in dPCR, NGS, and liquid biopsy applications.

Characterization

Characterized using various assays: Bioanalyzer HS DNA kit, all TapeStation kits, Qubit Fluorometer, dPCR, amplicon- and hybrid-captured-based targeted NGS, DNA methylation sequencing, and shallow WGS (data available upon reasonable request)

Source

HMEC-1 cells

Use cases

• Plasma spike-in for DNA extraction kit evaluation
• Internal Control for DNA size assessment
• Internal Control for DNA quantification

Product sizes

• ND-HMEC1-01_S: 500 ng in total (10 ng/µL in 50 µL buffer)
• ND-HMEC1-01_M: 2000 ng in total (10 ng/µL in 200 µL buffer)
• ND-HMEC1-01_L: 5000 ng in total (10 ng/µL in 500 µL buffer)

Formulation and storage

Tris-EDTA (10 mM Tris, 1 mM EDTA), pH 8.0

Storage at 2 - 8 °C

Product description

Highly characterized wild-type mono-nucleosomal (~166 bp) cfDNA for improved QC, preanalytical validation, and analytical validation in dPCR, NGS, and liquid biopsy applications.

Characterization

Characterized using various assays: Bioanalyzer HS DNA kit, all TapeStation kits, Qubit Fluorometer, dPCR, amplicon- and hybrid-captured-based targeted NGS, DNA methylation sequencing, and shallow WGS (data available upon reasonable request)

Source

HMEC-1 cells

Use cases

• Background DNA for Variant Allel Frequency (VAF) Validation
  • Negative control for: AKT1, ALK, APC, ATM, BRAF, BRCA1, CDKN2A, CTNNB1, EGFR, ERBB2, ESR1, FBXW7, FGFR1, FGFR2, FGFR3, GNA11, GNAQ, GNAS, HRAS, IDH1, IDH2, JAK2, KIT, KRAS, NF1, NRAS, NTRK3, PDGFRA, PIK3CA, POLE, PTCH1, PTEN, RET, STK11, TP53, TSC1, TSC2
  • Molecule counts for the following SNVs: ESR1 (L536H, Y537C, L536P, Y537S, L536R, D538G, E380Q, S463P, Y537N), BRAF V600E, BRAF K601E, KRAS G12D, KRAS G12C, KRAS G12V, KRAS G13D, NRAS G12S, NRAS G12D, NRAS Q61K, NRAS Q61R, PIK3CA H1047R, and PIK3CA E545K
• Plasma spike-in for DNA extraction kit evaluation
• Internal Control for DNA size assessment
• Internal Control for DNA quantification

Product sizes

• ND-HMEC1-02_S: 500 ng in total (10 ng/µL in 50 µL buffer)
• ND-HMEC1-02_M: 2000 ng in total (10 ng/µL in 200 µL buffer)
• ND-HMEC1-02_L: 5000 ng in total (10 ng/µL in 500 µL buffer)

Formulation and storage

Tris-EDTA (10 mM Tris, 1 mM EDTA), pH 8.0

Storage at 2 - 8 °C

Product description

Highly characterized long (1000 - 3000 bp) cfDNA for improved QC, preanalytical validation, and analytical validation in dPCR, NGS, and liquid biopsy applications.

Characterization

Characterized using various assays: Bioanalyzer HS DNA kit, all TapeStation kits, Qubit Fluorometer, dPCR, amplicon- and hybrid-captured-based targeted NGS, DNA methylation sequencing, and shallow WGS (data available upon reasonable request)

Source

PANC-1 cells

Use cases

• Control for long cfDNA characterization
• Plasma spike-in for DNA extraction kit evaluation
• Internal Control for DNA size assessment
• Internal Control for DNA quantification

Product sizes

• ND-PANC1-01_S: 500 ng in total (10 ng/µL in 50 µL buffer)
• ND-PANC1-01_M: 2000 ng in total (10 ng/µL in 200 µL buffer)
• ND-PANC1-01_L: 5000 ng in total (10 ng/µL in 500 µL buffer)

Formulation and storage

Tris-EDTA (10 mM Tris, 1 mM EDTA), pH 8.0

Storage at 2 - 8 °C

Product description

Highly characterized long cfDNA composed of various size populations for improved QC, preanalytical validation, and analytical validation in dPCR, NGS, and liquid biopsy applications.

Characterization

Characterized using various assays: Bioanalyzer HS DNA kit, all TapeStation kits, Qubit Fluorometer, dPCR, amplicon- and hybrid-captured-based targeted NGS, DNA methylation sequencing, and shallow WGS (data available upon reasonable request)

Source

PANC-1 cells

Use cases

• Control for long cfDNA characterization
• Plasma spike-in for DNA extraction kit evaluation
• Internal Control for DNA size assessment
• Internal Control for DNA quantification

Product sizes

• ND-PANC1-02_S: 500 ng in total (10 ng/µL in 50 µL buffer)
• ND-PANC1-02_M: 2000 ng in total (10 ng/µL in 200 µL buffer)
• ND-PANC1-02_L: 5000 ng in total (10 ng/µL in 500 µL buffer)

Formulation and storage

Tris-EDTA (10 mM Tris, 1 mM EDTA), pH 8.0

Storage at 2 - 8 °C